甘建华

基本信息

职称:研究员
电话:021-31246
543
邮箱:ganjhh@fudan.edu.cn
地址:复旦大学生命科学学院 A201-7室

 

个人简介

男,研究员,博导。1997年获北京医科大学药物化学学士学位。同年进入中国科学院上海有机化学研究所,师从中国早期著名的晶体学家夏宗芗教授,从事蛋白质晶体学方面的研究。 2002年获得有机所化学博士学位。在2003-2012年间先后以Postdoctoral Fellow,Research Associate 和Research Scientist 身份在美国国立卫生院(NIH)下属的癌症研究中心(NCI),美国纽约纪念斯隆凯特琳癌症中心及美国佐治亚州立大学进行研究。2012年9月受聘复旦大学生命科学学院研究员。

 

主要研究方向 (Research Interests)

以X-射线晶体学为主要手段,结合其他生物物理和生物化学方法研究生物大分子的结构和功能。主要包括:(1)RNA沉默通路中的核糖核酸酶的作用机制研究;(2)DNA复制和修复酶的结构和作用机制研究;3)具有催化功能的核酸分子的结构研究。

 

获奖情况 (Awards)

1. 获The SER-CAT Young Investigator Award (the Southeast Regional Collaborative Access Team,SER-CAT), USA, 2006.

2. 获FARE Award (The Fellows Award for Research Excellence, NIH), USA, 2007.

3. 获STTR Grant, USA, 2010-2011.

 

代表性成果

1. Y. Gao, H. Liu, C. Zhang, S. Su, Y. Chen, X. Chen, Y. Li, Z. Shao, Y. Zhang, Q. Shao, J. Li, Z. Huang, J. Ma, J. Gan*, “Structural basis for guide RNA trimming by RNase D ribonuclease in Trypanosoma brucei”, Nucleic Acids Res. (In press)

 

2. X. Chen, S. Su, Y. Chen, Y. Gao, Y. Li, Z. Shao, Y. Zhang, Q. Shao, H. Liu, J. Li, J. Ma, J. Gan*, “Structural studies reveal a ring-shaped architecture of deep-sea vent”, Nucleic Acids Res. 48(6): 3343-3355 (2020)

 

3. Y. Chen#, X. Chen#, Q. Huang, Z. Shao, Y. Gao, Y. Li, C. Yang, H. Liu, J. Li, Q. Wang, J. Ma, Y. Zhang*, Y, Gu*, J. Gan*, “A unique DNA-binding mode of African Swine fever virus AP endonuclease”, Cell Discovery, 6(13), doi:10.1038/s41421-020-0146-2 (2020)

 

4. Y. Zhang, H. Zhang, X. Xu, Y. Wang, W. Chen, Y. Wang, Z. Wu, N. Tang, Y. Wang, S. Zhao, J. Gan*, Q. Ji*, “Catalytic-state structure and engineering of Streptococcus thermophiles Cas9”, Nat Catal, doi: 10.1038/s41929-020-00506-9  (2020)

 

5. B. Wang#, Q. Luo#,Y. Li, L. Yin, N. Zhou, X Li, J. Gan*, A. Dong*, “Structural insights into target DNA recognition by R2R3-MYB transcription factors” Nucleic Acids Res. 48 (1): 460-471 (2020)

 

6. Y. Chen, H. Liu, C. Yang, Y. Gao, X. Yu, X. Chen, R. Cui, L. Zheng, S. Li, X. Li, J. Ma, Z. Huang*, J. Li*, J. Gan*, “Structure of the error-prone DNA ligase of African swine fever virus identifies critical active site residues”, Nat Commun. 10(1):387. doi: 10.1038/s41467-019-08296-w (2019)

 

7. H. Qian, H. Yu, P. Li, E. Zhu, Q. Yao, C. Tai, Z. Deng, K. Gerdes, X. He*, J. Gan*, H. Ou*, “Toxin-antitoxin operon kacAT of Klebsiella pneumoniae is regulated by conditional cooperativity via a W-shaped KacA-KacT complex”, Nucleic Acids Res. 47(14), 7690-7702 (2019)

 

8. W. Chen, H. Zhang, Y. Zhang, Y. Wang, J. Gan*, Q. Ji*, “Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nucleasePloS Biology 17(10):e3000496 (2019)

 

9. H. Liu, R. Wang, X. Yu, F. Shen, W. Lan, P. Haruehanroengra, Q. Yao, J. Zhang, Y. Chen, S. Li, B. Wu, L. Zheng, J. Ma, J. Lin, C. Cao*, J. Li*, J. Sheng*, J. Gan*, “High-resolution DNA quadruplex structure containing all the A-, G-, C-, T-tetrads”, Nucleic Acids Res. 46(21), 11627-11638 (2018)

 

10. C. Yang, R. Wu, H. Liu, Y. Chen, Y. Gao, X. Chen, Y. Li, J. Ma, J. Li*, J. Gan*, “Structural insights into DNA degradation by human mitochondrial nuclease MGME1”, Nucleic Acids Res. 46(20), 11075-11088 (2018)

 

11. H. Liu#, X. Yu#, Y. Chen, J. Zhang, B. Wu, L. Zheng, P. Haruehanroengra, R. Wang, S. Li, J. Lin, J. Li, J. Sheng, Z. Huang*, J. Ma*, J. Gan*, “Crystal structure of an RNA-cleaving DNAzyme”, Nat Commun. 8(1), doi: 10.1038/s41467-017-02203-x (2017)

 

12. H. Liu, F. Shen, P. Haruehanroengra, Q. Yao, Y. Cheng, Y. Chen, C. Yang, J. Zhang, B. Wu, Q. Luo, R. Cui, J. Li, J. Ma*, J. Sheng*, J. Gan*, “A DNA structure containing AgI-mediated G:G and C:C base pairs”, Angew Chem Int Ed Engl. 56(32):9430-9434 (2017)

 

13. H. Liu#, C. Chen#, P. Haruehanroengra, Q. Yao, Y. Chen, C. Yang, Q. Luo, B. Wu, J. Li, J. Ma*, J. Sheng*, J. Gan*, “Flexibility and stabilization of HgII-mediated C:T and T:T base pairs in DNA duplex”, Nucleic Acids Res, 45(5):2910-2918 (2017)

 

14. F. Shen, Z. Luo, H. Liu, R. Wang, S. Zhang, J. Gan*, J. Sheng*, “Structural insights into RNA duplexes with multipile 2’-5’-linkages”, Nucleic Acids Res. 45(6):3537-3546 (2017)

 

15. Y. Chen, J. Zhang, H. Liu, Y. Gao, X. Li, L. Zheng, R. Cui, Q. Yao, L. Rong, J. Li, Z. Huang*, J. Ma*, J. Gan*, “Unique 5’-P recognition and basis for dG:dGTP misincorporation of ASFV DNA polymerase X”, PloS Biology 15(2):e1002599 (2017)

 

16. J. Zhang, H. Liu, Q. Yao, X. Yu, Y. Chen, R. Cui, B. Wu, L. Zheng, J. Zuo, Z. Huang*, J. Ma*, J. Gan*, “Structural basis for single-stranded RNA recognition and cleavage by C3PO”, Nucleic Acids Res, 44(19):9494-9504 (2016)

 

17. J. Zhao#, X. Yu#, M. Zhu, H. Kang, J. Ma, M. Wu, J. Gan*, X. Deng*, H. Liang*, Structural and molecular mechanism of CdpR involved in quorum-sensing and bacterial virulence in Pseudomonas aeruginosa, PloS Biology, 14(4):e1002449 (2016).

 

18. J. Gan, J. Tropea, B. Austin, D. Court, D. Waugh, X. Ji*, “Structural Insight into the Mechanism of Double-Stranded RNA Processing by Ribonuclease III” Cell, 124(2), 355–366 (2006)

 

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