王永明

基本信息

职称:研究员

电话:021-31246624
邮箱:yonmiwang@gmail.com
地址:复旦大学生命科学学院 C623室

 

个人简介

博士,研究员,博士生导师。1997-2001年就读兰州大学生命科学学院并获得学士学位,2001-2004年就读东北师范大学生命科学学院并获得硕士学位,2005-2010年在德国马克斯-德尔布吕克分子医学中心(MDC)做博士生研究,并获得柏林自由大学博士学位。2010-2013年在斯坦福大学医学院从事博士后研究。2013年至今历任复旦大学生命科学学院青年研究员、研究员。

 

实验室链接:http://heartlab.fudan.edu.cn/

 

主要研究方向 (Research Interests)

1.建立高效的CRISPR/Cas9基因编辑技术;2.运用基因组编辑技术把基因突变引入到人体多潜能干细胞中制作心脏病模型,并通过这些模型研究先天性心脏病的发病机理和筛选治疗药物3.运用CRISPR/Cas9纠正人体的遗传突变,治疗疾病。

 

代表性成果

1. Xie YF*, Wang QZ*, Gao N, Wu FJ, Lan F, Zhang F, Jin L, Huang ZY, Ge JB#, Wang HY#, Wang YM#. MircroRNA-10b   promotes      human   embryonic    stem      cell-derived cardiomyocyte proliferation via a novel target gene LATS1. Molecular Therapy-Nucleic Acids 2019, in press

2. Wang B*, Wang R*, Wang DQ, Wu J, Li JX, Wang J, Liu HH, Wang YM#. Cas12aVDet: a CRISPR/Cas12a-based platform for rapid and visual nucleic acid detection. Analytical Chemistry 2019 Oct 1;91(19):12156-12161

3. Wang DQ*, Zhang CD*, Wang B, Li B, Wang Q, Liu D, Wang HY, Zhou Y, Shi LM, Lan F#, Wang YM#. Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning. Nature Communications 2019 Sep 19;10(1):4284

4.Li XW, Lu WJ, Li YN, Wu FJ, Bai R, Ma SH, Dong T, Zhang HJ, Lee A#, Wang YM#, Lan F#. MLP-deficient human pluripotent stem cell derived cardiomyocytes develop hypertrophic cardiomyopathy and heart failure phenotypes due to abnormal calcium handling. Cell Death & Disease 2019 Aug 13; 10(8):610

5. Xu F, Yang JC, Shi LM, Lan F, Shen L#, Wang YM#, Ge JB#. MicroRNA-302d promotes the proliferation of human pluripotent stem cell-derived cardiomyocytes by inhibiting LATS2 in the Hippo pathway. Clinical Science 2019 Jul 2;133(13):1387-1399

6. Fang X*, Miao SM*, Yu Y, Ding FY, Han XL, Wu HC, Zhao ZA, Wang YM, Hu SJ#, Lei W#. MIR148A family regulates cardiomyocyte differentiation of human embryonic stem cells by inhibiting the DLL1-mediated NOTCH signaling pathway. Journal of Molecular and Cellular Cardiology. 2019 Jun 22;134:1-12

7.Wang B, Wang ZS, Wang DQ, Zhang BL, Ong SG, Li MQ, Yu WQ, Wang YM. krCRISPR: an easy and efficient strategy for generating conditional knockout of essential genes in cells. Journal of Biological Engineering. 2019 Apr 24 eCollection 2019

8. Xie YF*, Ma AY*, Wang BS, Peng R, Jing YC, Wang DQ, Finnell RH, Qiao B, Wang YM#, Wang HY#, Zheng YF#. Rare mutations of ADAM17 from TOFs induce hypertrophy in human embryonic stem cell-derived cardiomyocytes via HB-EGF signaling. Clinical Science. 2019 Jan 22 133(2):225-238

9. Adam M, Kooreman NG,Jagger A, Wagenhäuser MU, Mehrkens D, Wang YM, Kayama Y, Toyama K, Raaz U, Schellinger IN, Maegdefessel L, Spin JM, Hamming JF, Quax PHA, Baldus S, Wu JC and Tsao PS. Systemic upregulation of IL-10 (Interleukin-10) using a nonimmunogenic vector reduces growth and rate of dissecting abdominal aortic aneurysm. Arterioscler Thromb Vasc Biol. 2018 Aug;38(8):1796-1805

10. Xie YF*, Wang DQ*, Lan F, Wei G, Ni T, Chai RJ, Liu D, Hu SJ, Li MQ, Li DJ, Wang HY, Wang YM. An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells. Scientific Reports. 2017 May24;7(1):2320

11. Wang YM*, Pryputniewicz-Dobrinska D*, Nagy EÉ*, Kaufman CD*, Singh M, Yant S, Wang J, Dalda A, Kay MA, Ivics Z, Izsvák Z. Regulated complex assembly safeguards the fidelity of Sleeping Beauty transposition. Nucleic Acids Research. 2017 Jan 9; 45(1):311-326 (IF: 8.2).

12. Qi JL*, Dong ZJ*, Shi YW, Wang X, Qin YY, Wang YM, Liu D. NgAgo-based fabp11a gene knockdown causes eye developmental defects in zebrafish. Cell Research. 2016 Dec; 26(12): 1349-1352 (IF: 14.8).

13. He ZH*, Sun S*, Waqas M, Zhang XL, Qian FP, Cheng C, Zhang MS, Zhang SS, Wang YM, Tang ML, Li HW, Chai RJ. Reduced TRMU expression increases the sensitivity of hair-cell-like HEI-OC-1 cells to neomycin damage in vitro. Scientific Reports. 2016 Jul; 13;6: 29621 (5.2).

14. Wang YM*, Liang P*, Lan F*, Wu HD*, Lisowski L, Gu MX, Hu SJ, Kay MA, Urnov FD, Shinnawi R, Gold J, Gepstein L, Wu JC. Genome editing of isogenic human induced pluripotent stem cells recapitulates long QT phenotype for drug testing. Journal of the American College of Cardiology. 2014 Aug 5; 64 (5): 451-459 (IF:14.1).

15. Wang YM*, Wang JC*, Chen JX, Deveraj A, Selbach M, Ivics Z and Izsvák Z. Suicidal autointegration of sleeping beauty and piggyBac transposons in eukaryotic cells. PloS Genetics. 2014 Mar13; 10(3): e10043103 (IF: 8.5).

16. Liang P*, Lan F*, Lee AS*, Gong TY, Sanchez-Freire V, Wang YM, Nguyen PK, Bers D, Robbins RC, Wu JC. Drug screening using a library of human induced pluripotent stem cell-derived cardiomyocytes reveals disease specific patterns of cardiotoxicity. Circulation. 2013 Apr; 127(16): 1677-91 (IF: 15.2).

17. Hu SJ, Wilson KD, Ghosh Z, Han L, Wang YM, Lan F, Ransohoff KJ, Wu JC. MicroRNA-302 increases reprogramming efficiency via repression of NR2F2. Stem Cells. 2013 Feb; 31(2):259-68 (IF: 7.7).

18. Lan F*, Lee AS*, Liang P*, Sanchez-Freire V, Nguyen PK, Wang L, Han L, Yen M, Wang YM, Sun N, Abilez OJ, Hu SJ, Ebert AD, Navarrete EG, Simmons CS, Wheeler M, Pruitt B, Lewis R, Yamaguchi Y, Ashley EA, Bers DM, Robbins RC, Longaker MT, Wu JC. Abnormal Calcium Handling Properties Underlie Pathogenesis of Familial Hypertrophic Cardiomyopathy in Patient-Specific Induced Pluripotent Stem Cells. Cell Stem Cell. 2013 Jan; 12(1) 101-13. (IF: 25.3).

19. Wang YM Zhang WY, Hu S, Lan F, Lee AS, Huber B, Lisowski L, Liang P, Huang M, de Almeida PE, Won JH, Sun N, Robbins RC, Kay MA, Urnov FD, Wu JC*.Genome editing of human embryonic stem cells and induced pluripotent stem cells with zinc finger nuclease for cellular imaging. Circulation Research. 2012 Dec 7; 111(12): 1494-503 (11.9).

20. Nguyen PK, Lan F, Wang YM, Wu JC. Imaging: Guiding the clinical translation of cardiac stem cell therapy. Circulation Research. 2011;109:962-979 (IF: 9.5).

21. Wang YM, Liška F*, Gosele C, Šedová L, Křen V, Křenová D, Ivics Z, Hubner N, and Izsvák Z. A novel active endogenous retrovirus family contributes to genome variability in rat inbred strains. Genome Research. 2010 Jan; 20(1) 19-27. (IF: 14.4).

 

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