学 术 报 告

Extracting function from the mouse genome

Professor Allan Bradley

Director of the Wellcome Trust Sanger Institute and a Fellow of the Royal Society

时 间：5月23日（星期二）下午4：20

地 点：立人生物楼107室

This is a specialized talk focus on the use of the mouse to functionally annotate the mouse genome. Professor Bradley will begin by introducing the history of ES cells and how these cells can be used as genetic vehicles to alter endogenous genes in the mouse genome. He will explain some of the technologies and how they have been utilized over the last 20 years and point out the limitations that are now apparent as a result of the human genome project. He will describe new initiatives to overcome these bottle necks, which sets the stage for future resources that will be generated in high throughput laboratories for the benefit community. Professor Bradley will describe some existing resources which enable the community to make large-scale alterations to the mouse genome such as deletions, duplications and balancers. In the latter part of the talk he will describe a genetic system which allows homozygous mutations to be generated in ES cells and describe how this had been used to identify genes involved in DNA repair and modulating resistance to retroviruses.

Summary:

 Embryonic stem cell technology

 Technology/throughput changes as a result of the mouse genome sequence

 Requirements for the post sequence analysis of gene function

 The KOMP/EUCOMM project

 The MICER resource

 Balancer chromosomes

 Generating homozygous mutations in ES cells

 Blm-deficient ES cells and gene trap libraries

 Screen for DNA mismatch repair and retroviral resistance genes

生命科学学院

2006年5月19日