王永明

发布者:王诗铭发布时间:2021-06-28浏览次数:583

教师基本信息

职称:研究员

电子邮箱:yonmiwang@gmail.com

办公地点:C623

办公电话:021-31246624


个人简介

王永明博士,复旦大学生命科学学院研究员。2001年本科毕业于兰州大学生命科学学院;2004硕士毕业于东北师范大学生命科学学院,导师为刘宝教授。2005-2010年在德国马克思戴尔布吕克分子医学中心(MDC)师从Zsuzsanna Izsvak教授研究睡美人转座子,取得了柏林自由大学博士学位。2010-2013年在斯坦福大学从事博士后研究,用基因编辑技术结合多能干细胞技术研究心肌病,导师为Joseph Wu教授。目前主要从事新型CRISPR工具开发与基因治疗研究。主持3项国家自然科学基金委面上项目,参与1项科技部重大研发项目。获得了2020年中国技术市场协会金桥奖个人奖和金龙鱼合成生物学学者奖。


研究方向

CRISPR技术及基因治疗


授课情况

《研究生专业英语》和本科生《生物科学研究设计与实践》。


招生专业

遗传学和分子生物学


代表性论文和论著

1. Hu ZY #, Zhang CD#, Wang S, Gao SQ, Wei JJ, Li MM, Hou LH, Mao HL, Wei YY, Qi T, Liu HM, Liu D, Lan F, Lu DR, Wang HY*, Li JX*, Wang YM*. Discovery and engineering of small SlugCas9 with broad targeting range and high specificity and activity. Nucleic Acids Research. 2021.

2. Li YN#, Chang Y#, Li XL, Li XW, Gao J, Zhou YF, Wu FJ, Bai R, Dong T, Ma SH, Zhang SY, Lu WJ, Wang YM*, Lan F*. RAD-deficient human cardiomyocytes develop hypertrophic cardiomyopathy phenotypes due to calcium dysregulation. Front Cell Dev Biol. Accepted.

3. Wang R#, Qian CY#, Pang YN, Li MM, Yang Y, Ma HJ, Zhao MY, Qian F, Yu H, Liu ZP, Ni T*, Zheng Y*, Wang YM*. opvCRISPR: one-pot visual RT-LAMP-CRISPR platform for SARS-CoV-2 detection. Biosensors & Bioelectronics. 2020 Oct 26;172:112766.

4. Han XL, Zhao ZA*, Yan SP, Lei W, Wu HC, Lu XA, Chen YQ, Li JJ, Wang YN, Yu M, Wang YM, Zheng YF, Wang HY, Shen ZY*, Hu SJ*. CXADR-like membrane protein protects against heart injury by preventing excessive pyroptosis after myocardial infarction. J Cell Mol Med. 2020 Oct 21.

5. Gao N, Zhang CD, Hu ZY, Li MM, Wei JJ, Wang YM*, Liu HH*. Characterization of Brevibacillus laterosporus Cas9 (BlatCas9) for mammalian genome editing. Front Cell Dev Biol. 2020 Oct 19.

6. Qi T#, Wu FJ#, Xie YQ#, Gao SQ, Li MM, Pu J, Li DL, Lan F*, Wang YM*. Base editing mediated generation of point mutations into human pluripotent stem cells for modeling disease. Front Cell Dev Biol. 2020; 8: 590581.

7. Chen ZJ, Li ZY, Hu XJ, Xie FY, Kuang SY, Zhan BW, Gao WQ, Chen XJ, Gao SQ, Li Y, Wang YM, Qian F, Ding C, Gan JH, Ji CN, Xu XW, Zhou Z, Huang JQ, He HH, Li JX. Structural basis of human helicase DDX21 in RNA binding, unwinding, and antiviral signal activation. Advance Science. 2020 Jun 8; 7(14):2000532.

8. Hu ZY, Wang S, Zhang CD, Gao N, Li MM, Wang DQ, Wang DQ, Liu D, Liu HH, Ong SG, Wang HY, Wang YM. A compact Cas9 ortholog from staphylococcus auricularis (SauriCas9) expands the DNA targeting scope. PLoS Biology. 2020 Mar; e3000686.

9. Wang B#, Xu MT#, Li MM, Wu FJ, Hu SJ, Chen XB, Zhao LQ, Huang ZY, Lan F*, Liu D*, Wang YM*. miR-25 promotes cardiomyocyte proliferation by targeting FBXW7. Molecular Therapy-Nucleic Acids. 2020 Mar 6; 19: 1299-1308.

10. Xie YF#, Wang QZ#, Gao n, Zhang F, Jin L, Huang ZY, Ge JB#, Wang HY*, Wang YM*. MircroRNA-10b promotes human embryonic stem cell-derived cardiomyocyte proliferation via a novel target gene LATS1. Molecular Therapy-Nucleic Acids. 2019 Nov 29; 19: 437-445.

11. Kesselring L, Miskey CZuliani C, Querques I, Kapitonov V, Laukó A, Fehér A, Palazzo A4, Diem T, Lustig JSebe AWang YM, Dinnyés A, Izsvák Z, Barabas O, Ivics. Z. A single amino acid switch converts the Sleeping Beauty transposase into an efficient unidirectional excisionase with utility in stem cell reprogramming. Nucleic Acids Research. 2019 Nov 28.

12. Wang B#, Wang R#, Wang DQ, Wu J, Li JX, Wang J, Liu HH, Wang YM. Cas12aVDet: a CRISPR/Cas12a-based platform for rapid and visual nucleic acid detection. Analytical Chemistry. 2019 Oct 1;91(19):12156-12161.

13. Wang DQ#, Zhang CD#, Wang B, Li B, WangQ, Liu D, Wang HY, Zhou Y, Shi LM, Lan F*, Wang YM*. Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning. Nature Communications. 2019 Sep 19;10(1):4284.

14. Li XW, Lu WJ, Li YN, Wu FJ, Bai R, Ma SH, Dong T, Zhang HJ, Lee A*, Wang YM*, Lan F*. MLP-deficient human pluripotent stem cell derived cardiomyocytes develop hypertrophic cardiomyopathy and heart failure phenotypes due to abnormal calcium handling. Cell Death & Disease. 2019 Aug 13; 10(8):610.

15. Xu F, Yang JC, Shi LM, Lan F, Shen L*, Wang YM*, Ge JB*. MicroRNA-302d promotes the proliferation of human pluripotent stem cell-derived cardiomyocytes by inhibiting LATS2 in the Hippo pathway. Clinical Science. 2019 Jul 2;133(13):1387-1399.

16. Fang X#, Miao SM#, Yu Y, Ding FY, Han XL, Wu HC, Zhao ZA, Wang YM, Hu SJ*, Lei W*. MIR148Afamilyregulatescardiomyocytedifferentiation of humanembryonicstemcells by inhibiting the DLL1-mediated NOTCH signaling pathway. Journal of Molecular and Cellular Cardiology. 2019 Jun 22;134:1-12.

17.Wang B, Wang ZS, Wang DQ, Zhang BL, Ong SG, Li MQ, Yu WQ, Wang YM. krCRISPR: an easy and efficient strategy for generating conditional knockout of essential genes in cells. Journal of Biological Engineering. 2019 Apr 24, eCollection 2019.

18. Xie YF#, Ma AY#, Wang BS, Peng R, Jing YC, Wang DQ, Finnell RH, Qiao B, Wang YM*, Wang HY*, Zheng YF*. Rare mutations of ADAM17 from TOFs induce hypertrophy in human embryonic stem cell-derived cardiomyocytes via HB-EGF signaling. Clinical Science. 2019 Jan 22 133(2):225-238.

19. Adam M#, Kooreman NG#, Jagger A, Wagenhäuser MU, Mehrkens D, Wang YM, Kayama Y, Toyama K, Raaz U, Schellinger IN, Maegdefessel L, Spin JM, Hamming JF, Quax PHA, Baldus S, Wu JC, Tsao PS. Systemic upregulation of IL-10 (Interleukin-10) using a nonimmunogenic vector reduces growth and rate of dissecting abdominal aortic aneurysm. Arterioscler Thromb Vasc Biol. 2018 Aug;38(8):1796-1805.

20. Yang RY, Wu YY, Ming Y, Xu YP, Wang SY, Shen JB, Wang CL, Chen X, Wang YM, Mao RF, Fan YH. A super-enhancer maintains homeostatic expression of Regnase-1. Gene. 2018 Aug 30;669:35-41.

21. Xie YF#, Wang DQ#, Lan F, Wei G, Ni T, Chai RJ, Liu D, Hu SJ, Li MQ, Li DJ, Wang HY*, Wang YM*. An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells. Scientific Reports. 2017 May24;7(1):2320.

22. Wang YM#, Pryputniewicz-Dobrinska D#, Nagy EÉ#, Kaufman CD#, Singh M, Yant S, Wang J, Dalda A, Kay MA, Ivics Z, Izsvák Z. Regulated complex assembly safeguards the fidelity of Sleeping Beauty transposition. Nucleic Acids Research. 2017 Jan 9; 45(1):311-326.

23. Qi JL#, Dong ZJ#, Shi YW, Wang X, Qin YY, Wang YM, Liu D. NgAgo-based fabp11a gene knockdown causes eye developmental defects in zebrafish. Cell Research. 2016 Dec; 26(12): 1349-1352.

24. He ZH#, Sun S#, Waqas M, Zhang XL, Qian FP, Cheng C, Zhang MS, Zhang SS, Wang YM, Tang ML, Li HW, Chai RJ. Reduced TRMU expression increases the sensitivity of hair-cell-like HEI-OC-1 cells to neomycin damage in vitro. Scientific Reports. 2016 Jul; 13;6: 29621.

25. Wang YM#, Liang P#, Lan F#, Wu HD#, Lisowski L, Gu MX, Hu SJ, Kay MA, Urnov FD, Shinnawi R, Gold J, Gepstein L, Wu JC. Genome editing of isogenic human induced pluripotent stem cells recapitulates long QT phenotype for drug testing. Journal of the American College of Cardiology. 2014 Aug 5; 64 (5): 451-459.

26. Wang YM#, Wang JC#, Chen JX, Deveraj A, Selbach M, Ivics Z and Izsvák Z. Suicidal autointegration of sleeping beauty and piggyBac transposons in eukaryotic cells. PloS Genetics. 2014 Mar13; 10(3): e10043103.

27. Liang P#, Lan F#, Lee AS#, Gong TY, Sanchez-Freire V, Wang YM, Nguyen PK, Bers D, Robbins RC, Wu JC. Drug screening using a library of human induced pluripotent stem cell-derived cardiomyocytes reveals disease specific patterns of cardiotoxicity. Circulation. 2013 Apr; 127(16): 1677-91.

28. Hu SJ, Wilson KD, Ghosh Z, Han L, Wang YM, Lan F, Ransohoff KJ, Wu JC. MicroRNA-302 increases reprogramming efficiency via repression of NR2F2. Stem Cells. 2013 Feb; 31(2):259-68.

29. Lan F#, Lee AS#, Liang P#, Sanchez-Freire V, Nguyen PK, Wang L, Han L, Yen M, Wang YM, Sun N, Abilez OJ, Hu SJ, Ebert AD, Navarrete EG, Simmons CS, Wheeler M, Pruitt B, Lewis R, Yamaguchi Y, Ashley EA, Bers DM, Robbins RC, Longaker MT, Wu JC. Abnormal calcium handling properties underlie pathogenesis of familial hypertrophic Cardiomyopathy in patient-specific induced pluripotent stem cells. Cell Stem Cell. 2013 Jan; 12(1) 101-13.

30. Wang YM#, Zhang WY#, Hu S, Lan F, Lee AS, Huber B, Lisowski L, Liang P, Huang M, de Almeida PE, Won JH, Sun N, Robbins RC, Kay MA, Urnov FD, Wu JC. Genome editing of human embryonic stem cells and induced pluripotent stem cells with zinc finger nuclease for cellular imaging. Circulation Research. 2012 Dec 7; 111(12): 1494-503.

31. Nguyen PK, Lan F, Wang YM, Wu JC. Imaging: Guiding the clinical translation of cardiac stem cell therapy. Circulation Research. 2011;109:962-979.

32. Wang YM#, Liška F#, Gosele C, Šedová L, Křen V, Křenová D, Ivics Z, Hubner N*, andIzsvák Z*.A novel active endogenous retrovirus family contributes to genome variability in rat inbred strains.Genome Research. 2010 Jan; 20(1) 19-27.