甘建华

发布时间：2021-06-27浏览次数：6107

教师基本信息

姓名：甘建华（Jianhua Gan）

职称：研究员（Professor）

电子邮箱：ganjhh@fudan.edu.cn

办公地点：生科院A201-7（Building of Life Sciences, Rm A201-7）

办公电话：021-31246543


研究方向

以X-射线晶体学为主要手段，结合其他生物物理和生物化学方法研究生物大分子的结构和功能。主要包括：（1）病原体关键蛋白的结构与功能研究；（2）DNA复制和修复酶的结构和作用机制研究；（3）具有催化功能的核酸分子的结构研究。

We mainly focus on the structural and functional studies of important macromolecules, including (1) pathogenesis-related proteins, (1) proteins involved in DNA replication and DNA repair pathways, and (3) nucleic acids with catalytic activities.


个人简介

研究员，博导。1997年获北京医科大学药物化学学士学位。同年进入中国科学院上海有机化学研究所，师从中国早期著名的晶体学家夏宗芗教授，从事蛋白质晶体学方面的研究。2002年获有机所化学博士学位。在2003-2012年间先后以Postdoctoral Fellow，Research Associate 和Research Scientist 身份在美国国立卫生院(NIH)下属的癌症研究中心(NCI)，美国纽约纪念斯隆凯特琳癌症中心及美国佐治亚州立大学进行研究。2006年荣获USA The SER-CAT Young Investigator Award （the Southeast Regional Collaborative Access Team，SER-CAT)；2007年获得USA FARE Award (The Fellows Award for Research Excellence, NIH)； 2010-2011年获得USA STTR Grant基金资助。2012年9月受聘复旦大学生命科学学院研究员。

EMPLOYMENT HISTORY

09/2012-present:    Professor, School of Life Sciences, Fudan University,  

Shanghai

06/2009-08/2012:   Research Scientist, Georgia State University, GA

04/2008-04/2009:   Research Associate, Memorial Sloan-Kettering Cancer

Center, NY

05/2003-04/2008:   Postdoctoral Fellow, NCI, National Institutes of Health,

Frederick, MD

08/2002-05/2003:   Research Associate, Shanghai Institute of Organic

Chemistry, Chinese Academy of Sciences, Shanghai

EDUCATION

09/1997-07/2002:   Ph.D in Organic Chemistry, Shanghai Institute of Organic

Chemistry, Chinese Academy of Sciences, Shanghai

09/1993-07/1997:   B.S. in Department of Pharmaceutical Chemistry, Beijing Medical University, Beijing

PROFESSIONAL ACTIVITY

Judge for the FARE 2008 Travel Award Competition, National Institutes of Health, Bethesda, USA (June, 2007)

AWARDS AND HONORS

STTR Research Award (2011)

The Fellows Award for Research Excellence (FARE), NIH, Bethesda, USA (2007)

The Spring Research Festival Poster Award, NCI-Fort Detrick, Frederick, USA (2007)

The SER-CAT Young Investigator Award, the Southeast Regional Collaborative Access Team (SER- CAT), USA (2006)

Exceptional Pay Increase, National Cancer Institute, NIH, USA (2006)

The Excellent Graduate Award, Beijing Medical University, Beijing, China (1997)

Peking Honor Graduate Award, Beijing, China (1997)


授课情况：生物化学荣誉课程（Biochemistry Honor Program）、结构生物学（Structural Biology）


招生专业：生物化学（Biochemistry）


科研项目：国家自然科学基金面上项目、科技部重点研发项目


代表性论文和论著：

  1. Z. Shao, S. Su, J. Yang, W. Zhang, Y. Gao, X. Zhao, Y. Zhang, Q. Shao, C. Cao, H. Li, H. Liu, J. Zhang, J. Ma, J. Gan*, “Structures and implications of the C962R protein of African swine fever virus”Nucleic Acids Res, 51(17): 9475-9490 (2023)

  2. H. Liu, Y. Gao, J. Mathivanan, Z. Armour-Garb, Z. Shao, Y. Zhang, X. Zhao, Q. Shao, W. Zhang, J. Yang, C. Cao, H. Li, J. Sheng*, J. Gan*, “Crystal structures and identification of novel Cd2+-specific DNA aptamer”Nucleic Acids Res, 51(9): 4625-4636 (2023)

  3. Z. Shao, J. Yang, Y. Gao, Y. Zhang, X. Zhao, Q. Shao, W. Zhang, C. Cao, H. Liu, J. Gan*, “Structural and functional studies of PCNA from African swine fever virus.” J. Virol, 97(8): e0074823 (2023)

  4. Y. Li, S. Su,Y. Gao, G. Lu, H. Liu, X. Chen, Z. Shao, Y. Zhang, Q. Shao, X. Zhao, J. Yang, C. Cao, J. Lin, J. Ma, J. Gan*,“Crystal structures and insights into precursor tRNA 5'-end processing by prokaryotic minimal protein-only RNase P” Nat Commun. 13(1):2290 (2022)

  5. Y. Zhang, Z. Shao, Y. Gao, B. Fan, J. Yang, X. Chen, X. Zhao, Q. Shao, W. Zhang, C. Cao, H. Liu, J. Gan*，“Structures and implications of the nuclease domain of human parvovirus B19 NS1 protein”, Comput Struct Biotec J, 20: 4645-4655 (2022)

  6. B. Wang, Q. Luo, Y. Li, K. Du, Z. Wu, T. Li, W. Shen, C. Huang*, J. Gan*, A. Dong*, “Structural insights into partner selection for MYB and bHLH transcription factor complexes” Nat Plants, 8(9): 1108-1117 (2022)

  7. R. Cui, H. Li, J. Zhao, X. Li, J. Gan*, J. Ma*, “Structural insights into the dual activities of the two-barrel RNA polymerase QDE-1”Nucleic Acids Res, 50(17): 10169-10186 (2022)

  8. Y. Xu, J. Yang, W. Li, S. Song, Y. S, L. Wu, J. Sun, Me. Hou, J. Wang, X. Jia, H. Zhang, M. Huang, T. Lu, J. Gan*, Y. Feng*, “Three enigmatic BioH isoenzymes are programmed in the early stage of mycobacterial biotin synthesis, an attractive anti-TB drug target” Plos Pathog, 18(7): e1010615 (2022)

  9. G. Cui, Y. Zhang, X. Xu, Y. Liu, Z. Li, M. Wu, J. Liu*, J. Gan*, H. Liang*, “PmiR senses 2-methylisocitrate levels to regulate bacterial virulence in Pseudomonas aeruginosa” Sci Adv, 8(49): eadd4220 (2022)

  10. Y. Gao, H. Liu, C. Zhang, S. Su, Y. Chen, X. Chen, Y. Li, Z. Shao, Y. Zhang, Q. Shao, J. Li, Z. Huang, J. Ma, J. Gan*, “Structural basis for guide RNA trimming by RNase D ribonuclease in Trypanosoma brucei”, Nucleic Acids Res. 49(1): 568-583 (2021)

  11. H. Jiang, Y. Gao, L. Zhang, D. Chen*, J. Gan*, A. Murchie*,“The identification and characterization of a selected SAM-dependent methyltransferase ribozyme that is present in natural sequences” Nat Catal, 4: 872-881 (2021)

  12. X. Chen, S. Su, Y. Chen, Y. Gao, Y. Li, Z. Shao, Y. Zhang, Q. Shao, H. Liu, J. Li, J. Ma, J. Gan*, “Structural studies reveal a ring-shaped architecture of deep-sea vent”, Nucleic Acids Res. 48(6): 3343-3355 (2020)

  13. Y. Chen, X. Chen, Q. Huang, Z. Shao, Y. Gao, Y. Li, C. Yang, H. Liu, J. Li, Q. Wang, J. Ma, Y. Zhang*, Y, Gu*, J. Gan*, “A unique DNA-binding mode of African Swine fever virus AP endonuclease”, Cell Discov, 6:13 (2020)

  14. Zhang, H. Zhang, X. Xu, Y. Wang, W. Chen, Y. Wang, Z. Wu, N. Tang, Y. Wang, S. Zhao, J. Gan*, Q. Ji*, “Catalytic-state structure and engineering of Streptococcus thermophiles Cas9”, Nat Catal, 3: 813-823 (2020)

  15. B. Wang, Q. Luo,Y. Li, L. Yin, N. Zhou, X Li, J. Gan*, A. Dong*, “Structural insights into target DNA recognition by R2R3-MYB transcription factors” Nucleic Acids Res. 48 (1): 460-471 (2020)

  16. Y. Chen, H. Liu, C. Yang, Y. Gao, X. Yu, X. Chen, R. Cui, L. Zheng, S. Li, X. Li, J. Ma, Z. Huang*, J. Li*, J. Gan*, “Structure of the error-prone DNA ligase of African swine fever virus identifies critical active site residues”, Nature Commun. 10(1):387 (2019)

  17. Qian, H. Yu, P. Li, E. Zhu, Q. Yao, C. Tai, Z. Deng, K. Gerdes, X. He*, J. Gan*, H. Ou*, “Toxin-antitoxin operon kacAT of Klebsiella pneumoniae is regulated by conditional cooperativity via a W-shaped KacA-KacT complex”, Nucleic Acids Res. 47(14): 7690-7702 (2019)

  18. W. Chen, H. Zhang, Y. Zhang, Y. Wang, J. Gan*, Q. Ji*, “Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease” PloS Biol 17(10):e3000496 (2019)

  19. H. Liu, R. Wang, X. Yu, F. Shen, W. Lan, P. Haruehanroengra, Q. Yao, J. Zhang, Y. Chen, S. Li, B. Wu, L. Zheng, J. Ma, J. Lin, C. Cao*, J. Li*, J. Sheng*, J. Gan*, “High-resolution DNA quadruplex structure containing all the A-, G-, C-, T-tetrads”, Nucleic Acids Res. 46(21): 11627-11638 (2018)

  20. C. Yang, R. Wu, H. Liu, Y. Chen, Y. Gao, X. Chen, Y. Li, J. Ma, J. Li*, J. Gan*, “Structural insights into DNA degradation by human mitochondrial nuclease MGME1”, Nucleic Acids Res. 46(20): 11075-11088 (2018)

  21. H. Liu#, X. Yu#, Y. Chen, J. Zhang, B. Wu, L. Zheng, P. Haruehanroengra, R. Wang, S. Li, J. Lin, J. Li, J. Sheng, Z. Huang*, J. Ma*, J. Gan*, “Crystal structure of an RNA-cleaving DNAzyme”, Nat Commun. 8(1): 2006 (2017)

  22. H. Liu, F. Shen, P. Haruehanroengra, Q. Yao, Y. Cheng, Y. Chen, C. Yang, J. Zhang, B. Wu, Q. Luo, R. Cui, J. Li, J. Ma*, J. Sheng*, J. Gan*, “A DNA structure containing AgI-mediated G:G and C:C base pairs”, Angew Chem Int Ed Engl. 56(32):9430-9434 (2017)

  23. H. Liu#, C. Chen#, P. Haruehanroengra, Q. Yao, Y. Chen, C. Yang, Q. Luo, B. Wu, J. Li, J. Ma*, J. Sheng*, J. Gan*, “Flexibility and stabilization of HgII-mediated C:T and T:T base pairs in DNA duplex”, Nucleic Acids Res, 45(5):2910-2918 (2017)

  24. F. Shen, Z. Luo, H. Liu, R. Wang, S. Zhang, J. Gan*, J. Sheng*, “Structural insights into RNA duplexes with multipile 2’-5’-linkages”, Nucleic Acids Res. 45(6):3537-3546 (2017)

  25. Y. Chen, J. Zhang, H. Liu, Y. Gao, X. Li, L. Zheng, R. Cui, Q. Yao, L. Rong, J. Li, Z. Huang*, J. Ma*, J. Gan*, “Unique 5’-P recognition and basis for dG:dGTP misincorporation of ASFV DNA polymerase X”, PloS Biol 15(2):e1002599 (2017)

  26. J. Zhang, H. Liu, Q. Yao, X. Yu, Y. Chen, R. Cui, B. Wu, L. Zheng, J. Zuo, Z. Huang*, J. Ma*, J. Gan*, “Structural basis for single-stranded RNA recognition and cleavage by C3PO”, Nucleic Acids Res, 44(19):9494-9504 (2016)

  27. J. Zhao#, X. Yu#, M. Zhu, H. Kang, J. Ma, M. Wu, J. Gan*, X. Deng*, H. Liang*, Structural and molecular mechanism of CdpR involved in quorum-sensing and bacterial virulence in Pseudomonas aeruginosa, PloS Biol, 14(4):e1002449 (2016).

  28. J. Gan, J. Tropea, B. Austin, D. Court, D. Waugh, X. Ji*, “Structural Insight into the Mechanism of Double-Stranded RNA Processing by Ribonuclease III” Cell, 124(2): 355–366 (2006)